Not Released
Sample In stock
Experience: Works
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Device

Part:BBa_K598022

Designed by: Yan GONG, Shuobin CHEN, Yunpeng LIU   Group: iGEM11_Peking_R   (2011-09-27)

Bistable Switch Mutant 42

The RBS of CI434 is CGAGACC in this bistble switch, which makes the RFP expresses three times as the GFP does in this bistable switch.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
    Illegal AgeI site found at 122
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2699


For the full characterisation of the device, please refer to BBa_K598002

[edit]
Categories
//chassis/prokaryote/ecoli
//direction/bidirectional
//plasmidbackbone/copynumber/high
Parameters
chassisE.coli DH5α
device_typeLogic circuit
emission509nm for eGFP and 610 for mRFP
excitation470nm for eGFP and 580nm for mRFP
n/aBistable Switch Mutant 42
resistanceChloramphenicol